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human lamp2  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank human lamp2
    Summary of antibodies employed in Western blot (WB) and immunofluorescence (IF) experiments.
    Human Lamp2, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lamp2+h4b4/anti-LAMP-2/pmc11117519-7-1-11
    Average 93 stars, based on 206 article reviews
    human lamp2 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Cholesterol Modulation Attenuates the AD-like Phenotype Induced by Herpes Simplex Virus Type 1 Infection"

    Article Title: Cholesterol Modulation Attenuates the AD-like Phenotype Induced by Herpes Simplex Virus Type 1 Infection

    Journal: Biomolecules

    doi: 10.3390/biom14050603

    Summary of antibodies employed in Western blot (WB) and immunofluorescence (IF) experiments.
    Figure Legend Snippet: Summary of antibodies employed in Western blot (WB) and immunofluorescence (IF) experiments.

    Techniques Used: Western Blot, Immunofluorescence, Infection, Plasmid Preparation

    HSV-1 infection induces intracellular cholesterol accumulation. ( A ) Intracellular cholesterol levels expressed as nanograms of cholesterol per micrograms of protein in SK-N-MC and N2a neuroblastoma cells treated with U18666A and water-soluble cholesterol. ( B ) Intracellular levels of cholesterol in cultures infected with HSV-1 at a multiplicity of infection (MOI) of 10 for 18 h compared to mock-infected cells. The graph data show the mean ± SEM of at least 4 independent experiments. Significance was recorded at p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***). ( C ) Confocal microscopy images of filipin staining (green) show cholesterol distribution patterns in SK-N-MC and N2a cells infected with HSV-1 (MOI 10) or treated with U18666A. TO-PRO-3-stained nuclei (red) are also shown. Immunofluorescence images of SK-N-MC cells uninfected ( D ) or infected with HSV-1 at MOI 10 ( E ) were obtained with filipin staining (green) and antibodies recognizing different markers (red) of early (EEA1) and late endosomes (CD222), autophagosomes (LC3), and lysosomes (LAMP2). TO-PRO-3-stained nuclei (blue) are also shown. Arrowheads in the merge panels showed the colocalization of filipin with CD222 and LC3 in HSV-1-infected cells. Scale bar: 10 µm.
    Figure Legend Snippet: HSV-1 infection induces intracellular cholesterol accumulation. ( A ) Intracellular cholesterol levels expressed as nanograms of cholesterol per micrograms of protein in SK-N-MC and N2a neuroblastoma cells treated with U18666A and water-soluble cholesterol. ( B ) Intracellular levels of cholesterol in cultures infected with HSV-1 at a multiplicity of infection (MOI) of 10 for 18 h compared to mock-infected cells. The graph data show the mean ± SEM of at least 4 independent experiments. Significance was recorded at p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***). ( C ) Confocal microscopy images of filipin staining (green) show cholesterol distribution patterns in SK-N-MC and N2a cells infected with HSV-1 (MOI 10) or treated with U18666A. TO-PRO-3-stained nuclei (red) are also shown. Immunofluorescence images of SK-N-MC cells uninfected ( D ) or infected with HSV-1 at MOI 10 ( E ) were obtained with filipin staining (green) and antibodies recognizing different markers (red) of early (EEA1) and late endosomes (CD222), autophagosomes (LC3), and lysosomes (LAMP2). TO-PRO-3-stained nuclei (blue) are also shown. Arrowheads in the merge panels showed the colocalization of filipin with CD222 and LC3 in HSV-1-infected cells. Scale bar: 10 µm.

    Techniques Used: Infection, Confocal Microscopy, Staining, Immunofluorescence

    Related Articles

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    Article Title: Hematopoietic Stem Cell Gene Therapy Alleviates Disease Phenotypes in a Murine Model of Danon Disease
    Article Snippet: Membranes were blocked with 5% non-fat dry milk in TBST and probed with antiLAMP2 (Abl 93; Developmental Studies Hybridoma Bank, 1:250; Iowa City, IA) or anti-LC3B (L7543, Sigma), human LAMP2 (H4B4) (Developmental Studies Hybridoma Bank); Rabbit anti-LC3B (Sigma), primary antibodies overnight at 40C, and re-probed with anti-GAPDH (GTX 100118, GeneTex) or GAPDH (Santa Cruz Biotechnology) as a loading standard.



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    Image Search Results


    Summary of antibodies employed in Western blot (WB) and immunofluorescence (IF) experiments.

    Journal: Biomolecules

    Article Title: Cholesterol Modulation Attenuates the AD-like Phenotype Induced by Herpes Simplex Virus Type 1 Infection

    doi: 10.3390/biom14050603

    Figure Lengend Snippet: Summary of antibodies employed in Western blot (WB) and immunofluorescence (IF) experiments.

    Article Snippet: , Human LAMP2 , Mouse , 1/1000 (WB) 1/50 (IF) , DSHB H4B4.

    Techniques: Western Blot, Immunofluorescence, Infection, Plasmid Preparation

    HSV-1 infection induces intracellular cholesterol accumulation. ( A ) Intracellular cholesterol levels expressed as nanograms of cholesterol per micrograms of protein in SK-N-MC and N2a neuroblastoma cells treated with U18666A and water-soluble cholesterol. ( B ) Intracellular levels of cholesterol in cultures infected with HSV-1 at a multiplicity of infection (MOI) of 10 for 18 h compared to mock-infected cells. The graph data show the mean ± SEM of at least 4 independent experiments. Significance was recorded at p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***). ( C ) Confocal microscopy images of filipin staining (green) show cholesterol distribution patterns in SK-N-MC and N2a cells infected with HSV-1 (MOI 10) or treated with U18666A. TO-PRO-3-stained nuclei (red) are also shown. Immunofluorescence images of SK-N-MC cells uninfected ( D ) or infected with HSV-1 at MOI 10 ( E ) were obtained with filipin staining (green) and antibodies recognizing different markers (red) of early (EEA1) and late endosomes (CD222), autophagosomes (LC3), and lysosomes (LAMP2). TO-PRO-3-stained nuclei (blue) are also shown. Arrowheads in the merge panels showed the colocalization of filipin with CD222 and LC3 in HSV-1-infected cells. Scale bar: 10 µm.

    Journal: Biomolecules

    Article Title: Cholesterol Modulation Attenuates the AD-like Phenotype Induced by Herpes Simplex Virus Type 1 Infection

    doi: 10.3390/biom14050603

    Figure Lengend Snippet: HSV-1 infection induces intracellular cholesterol accumulation. ( A ) Intracellular cholesterol levels expressed as nanograms of cholesterol per micrograms of protein in SK-N-MC and N2a neuroblastoma cells treated with U18666A and water-soluble cholesterol. ( B ) Intracellular levels of cholesterol in cultures infected with HSV-1 at a multiplicity of infection (MOI) of 10 for 18 h compared to mock-infected cells. The graph data show the mean ± SEM of at least 4 independent experiments. Significance was recorded at p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***). ( C ) Confocal microscopy images of filipin staining (green) show cholesterol distribution patterns in SK-N-MC and N2a cells infected with HSV-1 (MOI 10) or treated with U18666A. TO-PRO-3-stained nuclei (red) are also shown. Immunofluorescence images of SK-N-MC cells uninfected ( D ) or infected with HSV-1 at MOI 10 ( E ) were obtained with filipin staining (green) and antibodies recognizing different markers (red) of early (EEA1) and late endosomes (CD222), autophagosomes (LC3), and lysosomes (LAMP2). TO-PRO-3-stained nuclei (blue) are also shown. Arrowheads in the merge panels showed the colocalization of filipin with CD222 and LC3 in HSV-1-infected cells. Scale bar: 10 µm.

    Article Snippet: , Human LAMP2 , Mouse , 1/1000 (WB) 1/50 (IF) , DSHB H4B4.

    Techniques: Infection, Confocal Microscopy, Staining, Immunofluorescence